Genotyping of black grouse MHC class II B using reference Strand-Mediated Conformational Analysis (RSCA).

ABSTRACT:

Background

The Major Histocompatibility Complex (MHC) is a cluster of genes involved in the vertebrate immune system and includes loci with an extraordinary number of alleles. Due to the complex evolution of MHC genes, alleles from different loci within the same MHC class can be very similar and therefore difficult to assign to separate loci. Consequently, single locus amplification of MHC genes is hard to carry out in species with recently duplicated genes in the same MHC class, and multiple MHC loci have to be genotyped simultaneously. Since amplified alleles have the same length, accurate genotyping is difficult. Reference Strand-Mediated Conformational Analysis (RSCA), which is increasingly used in studies of natural populations with multiple MHC genes, is a genotyping method c

SUBMITTER: Strand TM 

PROVIDER: S-EPMC3141517 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature