TFRC and ACTB as the best reference genes to quantify Urokinase Plasminogen Activator in breast cancer.

ABSTRACT:

Background

Biomedical researchers have long looked for ways to diagnose and treat cancer patients at the early stages through biomarkers. Although conventional techniques are routinely applied in the detection of biomarkers, attitudes towards using Real-Time PCR techniques in detection of many biomarkers are increasing. Normalization of quantitative Real-Time PCR is necessary to validate non-biological alteration occurring during the steps of RNA quantification. Selection of variably expressed housekeeping genes (HKs) will affect the validity of the data. The aim of the present study was to identify uniformly expressed housekeeping genes in order to use in the breast cancer gene expression studies. Urokinase Plasminogen Activator was used as a gene of interest.

Findings

The

SUBMITTER: Majidzadeh-A K 

PROVIDER: S-EPMC3141519 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature