Identification of novel soybean microRNAs involved in abiotic and biotic stresses.

ABSTRACT:

Background

Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing.

Results

The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected

SUBMITTER: Kulcheski FR 

PROVIDER: S-EPMC3141666 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature