Multiple platform assessment of the EGF dependent transcriptome by microarray and deep tag sequencing analysis.

ABSTRACT:

Background

Epidermal Growth Factor (EGF) is a key regulatory growth factor activating many processes relevant to normal development and disease, affecting cell proliferation and survival. Here we use a combined approach to study the EGF dependent transcriptome of HeLa cells by using multiple long oligonucleotide based microarray platforms (from Agilent, Operon, and Illumina) in combination with digital gene expression profiling (DGE) with the Illumina Genome Analyzer.

Results

By applying a procedure for cross-platform data meta-analysis based on RankProd and GlobalAncova tests, we establish a well validated gene set with transcript levels altered after EGF treatment. We use this robust gene list to build higher order networks of gene interaction by interconnecting associated

SUBMITTER: Llorens F 

PROVIDER: S-EPMC3141672 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature