Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems.

ABSTRACT:

Background

Whole-mount in situ hybridization (WISH) is extensively used to characterize gene expression patterns in developing and adult brain and other tissues. To obtain an idea whether a novel gene might be involved in specification of a distinct brain subdivision, nucleus or neuronal lineage, it is often useful to correlate its expression with that of a known regional or neuronal marker gene. Two-color fluorescent in situ hybridization (FISH) can be used to compare different transcript distributions at cellular resolution. Conventional two-color FISH protocols require two separate rounds of horseradish peroxidase (POD)-based transcript detection, which involves tyramide signal amplification (TSA) and inactivation of the first applied antibody-enzyme conjugate before the second

SUBMITTER: Lauter G 

PROVIDER: S-EPMC3141750 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature