Renal cell carcinoma primary cultures maintain genomic and phenotypic profile of parental tumor tissues.

ABSTRACT:

Background

Clear cell renal cell carcinoma (ccRCC) is characterized by recurrent copy number alterations (CNAs) and loss of heterozygosity (LOH), which may have potential diagnostic and prognostic applications. Here, we explored whether ccRCC primary cultures, established from surgical tumor specimens, maintain the DNA profile of parental tumor tissues allowing a more confident CNAs and LOH discrimination with respect to the original tissues.

Methods

We established a collection of 9 phenotypically well-characterized ccRCC primary cell cultures. Using the Affymetrix SNP array technology, we performed the genome-wide copy number (CN) profiling of both cultures and corresponding tumor tissues. Global concordance for each culture/tissue pair was assayed evaluating the correlatio

SUBMITTER: Cifola I 

PROVIDER: S-EPMC3141767 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature