An effective virus-based gene silencing method for functional genomics studies in common bean.

ABSTRACT:

Background

Common bean (Phaseolus vulgaris L.) is a crop of economic and nutritious importance in many parts of the world. The lack of genomic resources have impeded the advancement of common bean genomics and thereby crop improvement. Although concerted efforts from the "Phaseomics" consortium have resulted in the development of several genomic resources, functional studies have continued to lag due to the recalcitrance of this crop for genetic transformation.

Results

Here we describe the use of a bean pod mottle virus (BPMV)-based vector for silencing of endogenous genes in common bean as well as for protein expression. This BPMV-based vector was originally developed for use in soybean. It has been successfully employed for both protein expression and gene silencing in thi

SUBMITTER: Diaz-Camino C 

PROVIDER: S-EPMC3141803 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature