Unravelling evolutionary strategies of yeast for improving galactose utilization through integrated systems level analysis.

ABSTRACT: Identification of the underlying molecular mechanisms for a derived phenotype by adaptive evolution is difficult. Here, we performed a systems-level inquiry into the metabolic changes occurring in the yeast Saccharomyces cerevisiae as a result of its adaptive evolution to increase its specific growth rate on galactose and related these changes to the acquired phenotypic properties. Three evolved mutants (62A, 62B, and 62C) with higher specific growth rates and faster specific galactose uptake were isolated. The evolved mutants were compared with a reference strain and two engineered strains, SO16 and PGM2, which also showed higher galactose uptake rate in previous studies. The profile of intermediates in galactose metabolism was similar in evolved and engineered mutants, whereas reserve ca

SUBMITTER: Hong KK 

PROVIDER: S-EPMC3141923 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature