Quantitative dissection of the simple repression input-output function.

ABSTRACT: We present a quantitative case study of transcriptional regulation in which we carry out a systematic dialogue between theory and measurement for an important and ubiquitous regulatory motif in bacteria, namely, that of simple repression. This architecture is realized by a single repressor binding site overlapping the promoter. From the theory point of view, this motif is described by a single gene regulation function based upon only a few parameters that are convenient theoretically and accessible experimentally. The usual approach is turned on its side by using the mathematical description of these regulatory motifs as a predictive tool to determine the number of repressors in a collection of strains with a large variation in repressor copy number. The predictions and corresponding measu

SUBMITTER: Garcia HG 

PROVIDER: S-EPMC3141941 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature