Superoxide production by macrophages and T cells is critical for the induction of autoreactivity and type 1 diabetes.

ABSTRACT:

Objective

The role of reactive oxygen species (ROS) and their dissipation in type 1 diabetes pathogenesis have garnered considerable controversy. Our recent work has demonstrated the importance of NADPH oxidase (NOX) activity for type 1 diabetes development and modulating T-cell autoreactivity. We previously linked decreased monocyte ROS with diabetes resistance in the alloxan-resistant mouse, and NOD-Ncf1(m1J) mice with a genetic ablation of NOX activity had reduced and delayed type 1 diabetes compared with NOD mice.

Research design and methods

To determine the required cellular sources of ROS that are necessary for type 1 diabetes initiation, we used antibody depletion and adoptive transfer experiments into NOD and NOD-Scid females, respectively. After receiving treatment,

SUBMITTER: Thayer TC 

PROVIDER: S-EPMC3142064 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature