Severity of diabetes governs vascular lipoprotein lipase by affecting enzyme dimerization and disassembly.

ABSTRACT:

Objective

In diabetes, when glucose consumption is restricted, the heart adapts to use fatty acid (FA) exclusively. The majority of FA provided to the heart comes from the breakdown of circulating triglyceride (TG), a process catalyzed by lipoprotein lipase (LPL) located at the vascular lumen. The objective of the current study was to determine the mechanisms behind LPL processing and breakdown after moderate and severe diabetes.

Research design and methods

To induce acute hyperglycemia, diazoxide, a selective, ATP-sensitive K(+) channel opener was used. For chronic diabetes, streptozotocin, a β-cell-specific toxin was administered at doses of 55 or 100 mg/kg to generate moderate and severe diabetes, respectively. Cardiac LPL processing into active dimers and breakdown at th

SUBMITTER: Wang Y 

PROVIDER: S-EPMC3142087 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature