A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study.

ABSTRACT:

Background

Mass spectrometry-based proteomics has reached a stage where it is possible to comprehensively analyze the whole proteome of a cell in one experiment. Here, the employment of stable isotopes has become a standard technique to yield relative abundance values of proteins. In recent times, more and more experiments are conducted that depict not only a static image of the up- or down-regulated proteins at a distinct time point but instead compare developmental stages of an organism or varying experimental conditions.

Results

Although the scientific questions behind these experiments are of course manifold, there are, nevertheless, two questions that commonly arise: 1) which proteins are differentially regulated regarding the selected experimental conditions, and 2) ar

SUBMITTER: Albaum SP 

PROVIDER: S-EPMC3142201 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature