Autophagy and p62 in cardiac proteinopathy.

ABSTRACT:

Rationale

Recent studies suggest an important role of autophagy in protection against αB-crystallin-based (CryAB(R120G)) desmin-related cardiomyopathies (DRC), but this has not been demonstrated in a different model of cardiac proteinopathy. Mechanisms underlying the response of cardiomyocytes to proteotoxic stress remain incompletely understood.

Objective

Our first objective was to determine whether and how the autophagic activity is changed in a mouse model of desminopathy. We also investigated the role of p62 in the protein quality control of cardiomyocytes.

Methods and results

Using an autophagosome reporter and determining changes in LC3-II protein levels in response to lysosomal inhibition, we found significantly increased autophagic flux in mouse hearts with tr

SUBMITTER: Zheng Q 

PROVIDER: S-EPMC3142307 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature