Fenoldopam preconditioning: role of heme oxygenase-1 in protecting human tubular cells and rodent kidneys against cold-hypoxic injury.

ABSTRACT:

Background

Kidneys from brain-dead donors are cold preserved until transplanted. However, prolonged cold storage can contribute to allograft failure. Studies suggest that donor preconditioning with dopaminergics may reduce cold-ischemic transplant injury, but whether heme oxygenase (HO)-1 induction is an underlying mechanism is not known.

Objective

To test whether preconditioning with fenoldopam (FD) induce HO-1 and protect kidneys against cold storage injury and whether HO-1 plays a role in protection.

Method

We used human renal proximal tubular epithelial cells, rat kidney transplants, and HO-1 null mice kidneys.

Results

FD preconditioning of cells for 4 hr significantly protected against cell death from 24-hr cold hypoxia and was associated with a dose-depen

SUBMITTER: Salahudeen AK 

PROVIDER: S-EPMC3142452 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature