Improving pan-genome annotation using whole genome multiple alignment.

ABSTRACT:

Background

Rapid annotation and comparisons of genomes from multiple isolates (pan-genomes) is becoming commonplace due to advances in sequencing technology. Genome annotations can contain inconsistencies and errors that hinder comparative analysis even within a single species. Tools are needed to compare and improve annotation quality across sets of closely related genomes.

Results

We introduce a new tool, Mugsy-Annotator, that identifies orthologs and evaluates annotation quality in prokaryotic genomes using whole genome multiple alignment. Mugsy-Annotator identifies anomalies in annotated gene structures, including inconsistently located translation initiation sites and disrupted genes due to draft genome sequencing or pseudogenes. An evaluation of species pan-genomes usi

SUBMITTER: Angiuoli SV 

PROVIDER: S-EPMC3142524 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature