The AvrM effector from flax rust has a structured C-terminal domain and interacts directly with the M resistance protein.

ABSTRACT: In plant immunity, recognition of pathogen effectors by plant resistance proteins leads to the activation of plant defenses and a localized cell death response. The AvrM effector from flax rust is a small secreted protein that is recognized by the M resistance protein in flax. Here, we investigate the mechanism of M-AvrM recognition and show that these two proteins directly interact in a yeast two-hybrid assay, and that this interaction correlates with the recognition specificity observed for each of the different AvrM variants. We further characterize this interaction by demonstrating that the C-terminal domain of AvrM is required for M-dependent cell death, and show that this domain also interacts with the M protein in yeast. We investigate the role of C-terminal differences among the di

SUBMITTER: Catanzariti AM 

PROVIDER: S-EPMC3142614 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature