Expression of HOXC8 is inversely related to the progression and metastasis of pancreatic ductal adenocarcinoma.

ABSTRACT:

Background

The transcription factor HOXC8 regulates many genes involved in tumour progression. This study was to investigate the role of HOXC8 in pancreatic ductal adenocarcinoma (PDAC) growth and metastasis.

Methods

The Hoxc8 expression was determined in 15 PDAC cell lines and human specimens by RT-polymerase chain reaction and/or immunohistochemistry. The effects of HOXC8 silencing by RNA interference were investigated by functional tests.

Results

The Hoxc8 mRNA expression in PDAC cell lines was negatively related to their growth in vivo. Except for Suit2-007 cells, only those with low Hoxc8 mRNA expression grew in nude rats. Successful down-regulation of HOXC8 expression caused increased proliferation, migration (P ≤ 0.05) and colony formation (P ≤ 0.05) in Suit2-0

SUBMITTER: Adwan H 

PROVIDER: S-EPMC3142801 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature