Construction of an American mink bacterial artificial chromosome (BAC) library and sequencing candidate genes important for the fur industry.

ABSTRACT:

Background

Bacterial artificial chromosome (BAC) libraries continue to be invaluable tools for the genomic analysis of complex organisms. Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects.

Results

Here, we report the construction and characterization of the CHORI-231 BAC library constructed from a Danish-farmed, male American mink (Neovison vison). The library contains approximately 165,888 clones with an average insert size of 170 kb, representing approximately 10-fold coverage. High-density filters, each consisting of 18,432 clones spotted in duplicate, have been produced for hybridization screening and are publicly available. Overgo probes derived from expressed sequence tags (ESTs

SUBMITTER: Anistoroaei R 

PROVIDER: S-EPMC3143106 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature