Quantitative proteomics identify novel miR-155 target proteins.

ABSTRACT:

Background

MicroRNAs are 22 nucleotides long non-coding RNAs and exert their function either by transcriptional or translational inhibition. Although many microRNA profiles in different tissues and disease states have already been discovered, only little is known about their target proteins. The microRNA miR-155 is deregulated in many diseases, including cancer, where it might function as an oncoMir.

Methodology/principal findings

We employed a proteomics technique called "stable isotope labelling by amino acids in cell culture" (SILAC) allowing relative quantification to reliably identify target proteins of miR-155. Using SILAC, we identified 46 putative miR-155 target proteins, some of which were previously reported. With luciferase reporter assays, CKAP5 was confirmed as

SUBMITTER: Lossner C 

PROVIDER: S-EPMC3143118 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature