Characterization of free-floating spheres from human trabecular meshwork (HTM) cell culture in vitro.

ABSTRACT: It has been observed in several tissues that direct isolation of cells in serum-free media and on nonadhesive substrates results in the formation of spherical clusters of cells known as free-floating spheres. Such free-floating spheres have been hypothesized to contain undifferentiated multipotent progenitor cells. Our goal was to isolate and characterize such free-floating spheres from HTM cell primary cultures. For this purpose, HTM cells were incubated in serum-free media and on a nonadhesive substrate. Individual free-floating spheres generated in these conditions were isolated in 96-well plates, and their proliferative capacity was evaluated by monitoring their size increase over time. The expression of the TM markers, MGP and CHI3L1, was examined using recombinant adenoviruses contai

SUBMITTER: Gonzalez P 

PROVIDER: S-EPMC3143838 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature