Candidate genes for non-diabetic ESRD in African Americans: a genome-wide association study using pooled DNA.

ABSTRACT: African Americans have increased susceptibility to non-diabetic (non-DM) forms of end-stage renal disease (ESRD) and extensive evidence supports a genetic contribution. A genome-wide association study (GWAS) using pooled DNA was performed in 1,000 African Americans to detect associated genes. DNA from 500 non-DM ESRD cases and 500 non-nephropathy controls was quantified using gel electrophoresis and spectrophotometric analysis and pools of 50 case and 50 control DNA samples were created. DNA pools were genotyped in duplicate on the Illumina HumanHap550-Duo BeadChip. Normalization methods were developed and applied to array intensity values to reduce inter-array variance. Allele frequencies were calculated from normalized channel intensities and compared between case and control pools. Thre

SUBMITTER: Bostrom MA 

PROVIDER: S-EPMC3144421 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature