Highly active engineered-enzyme oriented monolayers: formation, characterization and sensing applications.

ABSTRACT:

Background

The interest in introducing ecologically-clean, and efficient enzymes into modern industry has been growing steadily. However, difficulties associated with controlling their orientation, and maintaining their selectivity and reactivity is still a significant obstacle. We have developed precise immobilization of biomolecules, while retaining their native functionality, and report a new, fast, easy, and reliable procedure of protein immobilization, with the use of Adenylate kinase as a model system.

Methods

Self-assembled monolayers of hexane-1,6-dithiol were formed on gold surfaces. The monolayers were characterized by contact-angle measurements, Elman-reagent reaction, QCM, and XPS. A specifically designed, mutated Adenylate kinase, where cysteine was inserted at

SUBMITTER: Ulman A 

PROVIDER: S-EPMC3144448 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature