Enhanced biosurfactant production through cloning of three genes and role of esterase in biosurfactant release.

ABSTRACT:

Background

Biosurfactants have been reported to utilize a number of immiscible substrates and thereby facilitate the biodegradation of panoply of polyaromatic hydrocarbons. Olive oil is one such carbon source which has been explored by many researchers. However, studying the concomitant production of biosurfactant and esterase enzyme in the presence of olive oil in the Bacillus species and its recombinants is a relatively novel approach.

Results

Bacillus species isolated from endosulfan sprayed cashew plantation soil was cultivated on a number of hydrophobic substrates. Olive oil was found to be the best inducer of biosurfactant activity. The protein associated with the release of the biosurfactant was found to be an esterase. There was a twofold increase in the biosurfactan

SUBMITTER: Sekhon KK 

PROVIDER: S-EPMC3144451 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature