A latest and promising approach for prediction of viral load in hepatitis B virus infected patients.

ABSTRACT:

Introduction

Designing a rapid, reliable and sensitive assay for detection of hepatitis B virus (HBV) variants by real-time PCR is challenging at best. A recent approach for quantifying the viral load using a sensitive fluorescent principle was brushed in this study.

Materials and methods

: A total of 250 samples were collected from the outpatient unit, CLRD. Complete Human HBVDNA sequences (n = 944) were selected from the National Centre for Biotechnology Information (NCBI), primers and probes were designed and synthesized from the core, surface, and x region. Real-time based quantification was carried out using a standard kit and in-house generated standards and RT-PCR protocols.

Results and discussion

The standard calibration curve was generated by using serial dil

SUBMITTER: Yalamanchili N 

PROVIDER: S-EPMC3144682 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature