Identification and differential expression of microRNAs during metamorphosis of the Japanese flounder (Paralichthys olivaceus).

ABSTRACT:

Background

MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs of 20-25 nucleotides that play a key role in diverse biological processes. Japanese flounder undergo dramatic metamorphosis in their early development. The metamorphosis is characterized by morphological transformation from a bilaterally symmetrical to an asymmetrical body shape concomitant with extensive morphological and physiological remodeling of organs. So far, only a few miRNAs have been identified in fish and there are very few reports about the Japanese flounder miRNA.

Methodology/principal findings

Solexa sequencing technology was used to perform high throughput sequencing of the small RNA library from the metamorphic period of Japanese flounder. Subsequently, aligning these sequencing dat

SUBMITTER: Fu Y 

PROVIDER: S-EPMC3144956 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature