Secretory mediators regulate Nod2-induced tolerance in human macrophages.

ABSTRACT:

Background & aims

Nucleotide oligomerization domain 2 (Nod2) polymorphisms increase the risk of developing Crohn's disease, which is characterized by chronic intestinal inflammation. Bacterial peptidoglycan products chronically stimulate Nod2 in the intestine. Recent studies found that chronic Nod2 stimulation in human macrophages down-regulates proinflammatory cytokines on Nod2 or Toll-like receptor (TLR) restimulation. Therefore, an emerging hypothesis is that Nod2-mediated cytokine down-regulation is required for intestinal homeostasis, but the mechanisms mediating this down-regulation are incompletely understood.

Methods

Utilizing primary human macrophages, we examined secretory mediators as a mechanism of Nod2-mediated tolerance by inhibiting their function and assessin

SUBMITTER: Hedl M 

PROVIDER: S-EPMC3145247 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature