Gene discovery using next-generation pyrosequencing to develop ESTs for Phalaenopsis orchids.

ABSTRACT:

Background

Orchids are one of the most diversified angiosperms, but few genomic resources are available for these non-model plants. In addition to the ecological significance, Phalaenopsis has been considered as an economically important floriculture industry worldwide. We aimed to use massively parallel 454 pyrosequencing for a global characterization of the Phalaenopsis transcriptome.

Results

To maximize sequence diversity, we pooled RNA from 10 samples of different tissues, various developmental stages, and biotic- or abiotic-stressed plants. We obtained 206,960 expressed sequence tags (ESTs) with an average read length of 228 bp. These reads were assembled into 8,233 contigs and 34,630 singletons. The unigenes were searched against the NCBI non-redundant (NR) protein dat

SUBMITTER: Hsiao YY 

PROVIDER: S-EPMC3146457 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature