Evaluating the stoichiometry of macromolecular complexes using multisignal sedimentation velocity.

ABSTRACT: Gleaning information regarding the molecular physiology of macromolecular complexes requires knowledge of their component stoichiometries. In this work, a relatively new means of analyzing sedimentation velocity (SV) data from the analytical ultracentrifuge is examined in detail. The method depends on collecting concentration profile data simultaneously using multiple signals, like Rayleigh interferometry and UV spectrophotometry. If the cosedimenting components of a complex are spectrally distinguishable, continuous sedimentation-coefficient distributions specific for each component can be calculated to reveal the molar ratio of the complex's components. When combined with the hydrodynamic information available from the SV data, a stoichiometry can be derived. Herein, the spectral propert

SUBMITTER: Padrick SB 

PROVIDER: S-EPMC3147156 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature