Dataset Information
In vivo study in Trypanosoma brucei links mitochondrial transfer RNA import to mitochondrial protein import.
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ABSTRACT:
SUBMITTER: Tschopp F
PROVIDER: S-EPMC3147259 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Publications
In vivo study in Trypanosoma brucei links mitochondrial transfer RNA import to mitochondrial protein import.
EMBO reports 20110701 8
Trypanosoma brucei imports all mitochondrial transfer RNAs (tRNAs) from the cytosol. By using cell lines that allow independent tetracycline-inducible RNA interference and isopropyl-β-D-thiogalactopyranoside-inducible expression of a tagged tRNA, we show that ablation of Tim17 and mitochondrial heat-shock protein 70, components of the inner-membrane protein translocation machinery, strongly inhibits import of newly synthesized tRNAs. These findings, together with previous results in yeast and pl ...[more]