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Development of a 16S rRNA gene primer and PCR-restriction fragment length polymorphism method for rapid detection of members of the genus Megasphaera and species-level identification.

ABSTRACT: The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members of Megasphaera. Direct detection and identification were achieved for environmental samples and isolates.

SUBMITTER: Ohnishi A 

PROVIDER: S-EPMC3147432 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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Development of a 16S rRNA gene primer and PCR-restriction fragment length polymorphism method for rapid detection of members of the genus Megasphaera and species-level identification.

Ohnishi Akihiro A   Abe Shinko S   Nashirozawa Shiho S   Shimada Sayaka S   Fujimoto Naoshi N   Suzuki Masaharu M  

Applied and environmental microbiology 20110624 15

The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members of Megasphaera. Direct detection and identification were achieved for environmental samples and isolates. ...[more]

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