Development of a method for markerless gene deletion in Pseudomonas putida.

ABSTRACT: We developed a negative counterselection system for Pseudomonas putida based on uracil phosphoribosyltransferase (UPRTase) and sensitivity against the antimetabolite 5-fluorouracil (5-FU). We constructed a P. putida strain that is resistant to 5-FU and constructed vectors for the deletion of the surface adhesion protein gene, the flagellum biosynthesis operon, and two endonuclease genes. The genes were efficiently disrupted and left a markerless chromosomal in-frame deletion.

SUBMITTER: Graf N 

PROVIDER: S-EPMC3147467 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature