Mechanical disruption of lysis-resistant bacterial cells by use of a miniature, low-power, disposable device.

ABSTRACT: Molecular detection of microorganisms requires microbial cell disruption to release nucleic acids. Sensitive detection of thick-walled microorganisms such as Bacillus spores and Mycobacterium cells typically necessitates mechanical disruption through bead beating or sonication, using benchtop instruments that require line power. Miniaturized, low-power, battery-operated devices are needed to facilitate mechanical pathogen disruption for nucleic acid testing at the point of care and in field settings. We assessed the lysis efficiency of a very small disposable bead blender called OmniLyse relative to the industry standard benchtop Biospec Mini-BeadBeater. The OmniLyse weighs approximately 3 g, at a size of approximately 1.1 cm(3) without the battery pack. Both instruments were used to mecha

SUBMITTER: Vandeventer PE 

PROVIDER: S-EPMC3147885 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature