Characterization of glycosylation profiles of HIV-1 transmitted/founder envelopes by mass spectrometry.

ABSTRACT: The analysis of HIV-1 envelope carbohydrates is critical to understanding their roles in HIV-1 transmission as well as in binding of envelope to HIV-1 antibodies. However, direct analysis of protein glycosylation by glycopeptide-based mass mapping approaches involves structural simplification of proteins with the use of a protease followed by an isolation and/or enrichment step before mass analysis. The successful completion of glycosylation analysis is still a major analytical challenge due to the complexity of samples, wide dynamic range of glycopeptide concentrations, and glycosylation heterogeneity. Here, we use a novel experimental workflow that includes an up-front complete or partial enzymatic deglycosylation step before trypsin digestion to characterize the glycosylation patterns a

SUBMITTER: Go EP 

PROVIDER: S-EPMC3147976 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature