Development of a 950-gene DNA array for examining gene expression patterns in mouse testis.

ABSTRACT:

Background

Over the past five years, interest in and use of DNA array technology has increased dramatically, and there has been a surge in demand for different types of arrays. Although manufacturers offer a number of pre-made arrays, these are generally of utilitarian design and often cannot accommodate the specific requirements of focused research, such as a particular set of genes from a particular tissue. We found that suppliers did not provide an array to suit our particular interest in testicular toxicology, and therefore elected to design and produce our own.

Results

We describe the procedures used by members of the US Environmental Protection Agency MicroArray Consortium (EPAMAC) to produce a mouse testis expression array on both filter and glass-slide formats. The a

SUBMITTER: Rockett JC 

PROVIDER: S-EPMC31483 | biostudies-literature | 2001

REPOSITORIES: biostudies-literature