Predator-induced defences in Daphnia pulex: selection and evaluation of internal reference genes for gene expression studies with real-time PCR.

ABSTRACT:

Background

The planktonic microcrustacean Daphnia pulex is among the best-studied animals in ecological, toxicological and evolutionary research. One aspect that has sustained interest in the study system is the ability of D. pulex to develop inducible defence structures when exposed to predators, such as the phantom midge larvae Chaoborus. The available draft genome sequence for D. pulex is accelerating research to identify genes that confer plastic phenotypes that are regularly cued by environmental stimuli. Yet for quantifying gene expression levels, no experimentally validated set of internal control genes exists for the accurate normalization of qRT-PCR data.

Results

In this study, we tested six candidate reference genes for normalizing transcription levels of D. pulex

SUBMITTER: Spanier KI 

PROVIDER: S-EPMC3148505 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature