Array-based genomic screening at diagnosis and during follow-up in chronic lymphocytic leukemia.

ABSTRACT:

Background

High-resolution genomic microarrays enable simultaneous detection of copy-number aberrations such as the known recurrent aberrations in chronic lymphocytic leukemia [del(11q), del(13q), del(17p) and trisomy 12], and copy-number neutral loss of heterozygosity. Moreover, comparison of genomic profiles from sequential patients' samples allows detection of clonal evolution.

Design and methods

We screened samples from 369 patients with newly diagnosed chronic lymphocytic leukemia from a population-based cohort using 250K single nucleotide polymorphism-arrays. Clonal evolution was evaluated in 59 follow-up samples obtained after 5-9 years.

Results

At diagnosis, copy-number aberrations were identified in 90% of patients; 70% carried known recurrent alterations, in

SUBMITTER: Gunnarsson R 

PROVIDER: S-EPMC3148910 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature