Phenotypic and functional evaluation of CD3+CD4-CD8- T cells in human CD8 immunodeficiency.

ABSTRACT:

Background

Human CD8 immunodeficiency is characterized by undetectable CD8(+) lymphocytes and an increased population of CD4(-)CD8(-) (double negative) T lymphocytes.

Design and methods

We hypothesized that the double negative subset corresponds to the cellular population that should express CD8 and is committed to the cytotoxic T lymphocyte lineage. To assess this, we determined the phenotype and function of peripheral blood mononuclear cells and/or magnetically isolated double negative T lymphocytes from two CD8-deficient patients. To analyze the expression and co-localization with different organelles, 293T cells were transfected with plasmids bearing wild-type or mutated CD8α.

Results

CD8α mutated protein was retained in the cytoplasm of transfected cells. The per

SUBMITTER: Bernardo I 

PROVIDER: S-EPMC3148914 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature