Genome-wide detection of chromosomal rearrangements, indels, and mutations in circular chromosomes by short read sequencing.

ABSTRACT: Whole-genome sequencing (WGS) with new short-read sequencing technologies has recently been applied for genome-wide identification of mutations. Genomic rearrangements have, however, often remained undetected by WGS, and additional analyses are required for their detection. Here, we have applied a combination of WGS and genome copy number analysis, for the identification of mutations that suppress the growth deficiency imposed by excessive initiations from the Escherichia coli origin of replication, oriC. The E. coli chromosome, like the majority of bacterial chromosomes, is circular, and DNA replication is initiated by assembling two replication complexes at the origin, oriC. These complexes then replicate the chromosome bidirectionally toward the terminus, ter. In a population of growing

SUBMITTER: Skovgaard O 

PROVIDER: S-EPMC3149504 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature