A multiplex real-time PCR assay targeting virulence and resistance genes in Salmonella enterica serotype Typhimurium.

ABSTRACT:

Background

Typhimurium is the main serotype of Salmonella enterica subsp. enterica implicated in food-borne diseases worldwide. This study aimed to detect the prevalence of ten markers combined in a macro-array based on multiplex real-time PCR. We targeted characteristic determinants located on pathogenicity islands (SPI-2 to -5, virulence plasmid pSLT and Salmonella genomic island 1 (SGI1)) as well as a specific 16S-23S rRNA intergenic spacer sequence of definitive type 104 (DT104). To investigate antimicrobial resistance, the study also targeted the presence of genes involved in sulfonamide (sul1) and beta-lactam (blaTEM) resistance. Finally, the intI1 determinant encoding integrase from class 1 integron was also investigated.

Results

A total of 538 unrelated S. Typhimuriu

SUBMITTER: Bugarel M 

PROVIDER: S-EPMC3150258 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature