Boltzmann energy-based image analysis demonstrates that extracellular domain size differences explain protein segregation at immune synapses.

ABSTRACT: Immune synapses formed by T and NK cells both show segregation of the integrin ICAM1 from other proteins such as CD2 (T cell) or KIR (NK cell). However, the mechanism by which these proteins segregate remains unclear; one key hypothesis is a redistribution based on protein size. Simulations of this mechanism qualitatively reproduce observed segregation patterns, but only in certain parameter regimes. Verifying that these parameter constraints in fact hold has not been possible to date, this requiring a quantitative coupling of theory to experimental data. Here, we address this challenge, developing a new methodology for analysing and quantifying image data and its integration with biophysical models. Specifically we fit a binding kinetics model to 2 colour fluorescence data for cytoskeleto

SUBMITTER: Burroughs NJ 

PROVIDER: S-EPMC3150282 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature