Activation of corticotropin-releasing factor receptor 2 mediates the colonic motor coping response to acute stress in rodents.

ABSTRACT:

Background & aims

Corticotropin-releasing factor receptor-1 (CRF(1)) mediates the stress-induced colonic motor activity. Less is known about the role of CRF(2) in the colonic response to stress.

Methods

We studied colonic contractile activity in rats and CRF(2)-/-, CRF-overexpressing, and wild-type mice using still manometry; we analyzed defecation induced by acute partial-restraint stress (PRS), and/or intraperitoneal injection of CRF ligands. In rats, we monitored activation of the colonic longitudinal muscle myenteric plexus (LMMP) neurons and localization of CRF(1) and CRF(2) using immunohistochemical and immunoblot analyses. We measured phosphorylation of extracellular signal-regulated kinase 1/2 by CRF ligands in primary cultures of LMMP neurons (PC-LMMPn) and cyclic a

SUBMITTER: Gourcerol G 

PROVIDER: S-EPMC3150547 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature