Detection of transgene in early developmental stage by GFP monitoring enhances the efficiency of genetic transformation of pepper.

ABSTRACT: In order to establish a reliable and highly efficient method for genetic transformation of pepper, a monitoring system featuring GFP (green fluorescent protein) as a report marker was applied to Agrobacterium-mediated transformation. A callus-induced transformation (CIT) system was used to transform the GFP gene. GFP expression was observed in all tissues of T(0), T(1) and T(2) peppers, constituting the first instance in which the whole pepper plant has exhibited GFP fluorescence. A total of 38 T(0) peppers were obtained from 4,200 explants. The transformation rate ranged from 0.47 to 1.83% depending on the genotype, which was higher than that obtained by CIT without the GFP monitoring system. This technique could enhance selection power by monitoring GFP expression at the early stage of c

SUBMITTER: Jung M 

PROVIDER: S-EPMC3150808 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature