Conformational bias imposed by source microseeds results in structural ambiguity.

ABSTRACT: The p38 MAP kinase pathway is an essential component of numerous cellular signalling networks which are usually activated in response to extracellular environmental stress conditions. In addition to the canonical activation, several alternative activation pathways have been identified for p38; one of these, in which p38 is initially phosphorylated on Tyr323 and consequently autoactivated, is exclusive to T cells and is induced by TCR activation. Intrinsically active and inactive mutants at position 323 have been developed in order to evaluate the structural changes that occur upon TCR-induced activation. In order to promote crystal growth, cross streak-seeding techniques were utilized. This technique has gained popularity in promoting crystal growth when spontaneous nucleation induces crit

SUBMITTER: Tzarum N 

PROVIDER: S-EPMC3151118 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature