Methodologies for the characterization, identification and quantification of S-nitrosylated proteins.

ABSTRACT:

Background

Protein S-nitrosylation plays a central role in signal transduction by nitric oxide (NO), and aberrant S-nitrosylation of specific proteins is increasingly implicated in disease.

Scope of review

Here, methodologies for the characterization, identification and quantification of SNO-proteins are reviewed, focusing on techniques suitable for the structural characterization and absolute quantification of isolated SNO-proteins, the identification and relative quantification of SNO-proteins from complex mixtures as well as the mass spectrometry-based identification and relative quantification of sites of S-nitrosylation (SNO-sites) in proteins.

Major conclusions

Structural characterization of SNO-proteins by X-ray crystallography is increasingly being utilized to

SUBMITTER: Foster MW 

PROVIDER: S-EPMC3151544 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature