High-throughput analysis of the mutagenic and cytotoxic properties of DNA lesions by next-generation sequencing.

ABSTRACT: Human cells are constantly exposed to environmental and endogenous agents which can induce damage to DNA. Understanding the implications of these DNA modifications in the etiology of human diseases requires the examination about how these DNA lesions block DNA replication and induce mutations in cells. All previously reported shuttle vector-based methods for investigating the cytotoxic and mutagenic properties of DNA lesions in cells have low-throughput, where plasmids containing individual lesions are transfected into cells one lesion at a time and the products from the replication of individual lesions are analyzed separately. The advent of next-generation sequencing (NGS) technology has facilitated investigators to design scientific approaches that were previously not technically feasib

SUBMITTER: Yuan B 

PROVIDER: S-EPMC3152323 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature