A DNA minor groove electronegative potential genome map based on photo-chemical probing.

ABSTRACT: The double-stranded DNA of the genome contains both sequence information directly relating to the protein and RNA coding as well as functional and structural information relating to protein recognition. Only recently is the importance of DNA shape in this recognition process being fully appreciated, and it also appears that minor groove electronegative potential may contribute significantly in guiding proteins to their cognate binding sites in the genome. Based on the photo-chemical probing results, we have derived an algorithm that predicts the minor groove electronegative potential in a DNA helix of any given sequence. We have validated this model on a series of protein-DNA binding sites known to involve minor groove electrostatic recognition as well as on stable nucleosome core complexe

SUBMITTER: Lindemose S 

PROVIDER: S-EPMC3152351 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature