Myocyte specific overexpression of myoglobin impairs angiogenesis after hind-limb ischemia.

ABSTRACT:

Objective

In preclinical models of peripheral arterial disease the angiogenic response is typically robust, though it can be impaired in conditions such as hypercholesterolemia and diabetes where the endothelium is dysfunctional. Myoglobin (Mb) is expressed exclusively in striated muscle cells. We hypothesized that myocyte specific overexpression of myoglobin attenuates ischemia-induced angiogenesis even in the presence of normal endothelium.

Methods and results

Mb overexpressing transgenic (MbTg, n=59) and wild-type (WT, n=56) C57Bl/6 mice underwent unilateral femoral artery ligation/excision. Perfusion recovery was monitored using Laser Doppler. Ischemia-induced changes in muscle were assessed by protein and immunohistochemistry assays. Nitrite/nitrate and protein-bound NO

SUBMITTER: Hazarika S 

PROVIDER: S-EPMC3152376 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature