Expression of core antigen of HCV genotype 3a and its evaluation as screening agent for HCV infection in Pakistan.

ABSTRACT:

Background

Pakistan is facing a threat from hepatitis C infection which is increasing at an alarming rate throughout the country. More specific and sensitive screening assays are needed to timely and correctly diagnose this infection.

Methods

After RNA extraction from specimen (HCV-3a), cDNA was synthesized that was used to amplify full length core gene of HCV 3a. After verification through PCR, DNA sequencing and BLAST, a properly oriented positive recombinant plasmid for core gene was digested with proper restriction enzymes to release the target gene which was then inserted downstream of GST encoding DNA in the same open reading frame at proper restriction sites in multiple cloning site of pGEX4t2 expression vector. Recombinant expression vector for each gene was transfor

SUBMITTER: Yousaf MZ 

PROVIDER: S-EPMC3152539 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature