Expression level of Hand2 affects specification of enteric neurons and gastrointestinal function in mice.

ABSTRACT:

Background & aims

Hand2 is a basic helix-loop-helix transcription factor required for terminal differentiation of enteric neurons. We studied Hand2 haploinsufficient mice, to determine whether reduced expression of Hand2 allows sufficient enteric neurogenesis for survival, but not for development of a normal enteric nervous system (ENS).

Methods

Enteric transcripts that encode Hand2 and the neuron-specific embryonic lethal abnormal vision proteins HuB, HuC, and HuD were quantified. Immunocytochemistry was used to identify and quantify neurons. Apoptosis was analyzed with the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling procedure. Intracellular microelectrodes were used to record inhibitory junction potentials. Gastrointestinal tr

SUBMITTER: D'Autreaux F 

PROVIDER: S-EPMC3152642 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature