Coquillettidia (Culicidae, Diptera) mosquitoes are natural vectors of avian malaria in Africa.

ABSTRACT:

Background

The mosquito vectors of Plasmodium spp. have largely been overlooked in studies of ecology and evolution of avian malaria and other vertebrates in wildlife.

Methods

Plasmodium DNA from wild-caught Coquillettidia spp. collected from lowland forests in Cameroon was isolated and sequenced using nested PCR. Female Coquillettidia aurites were also dissected and salivary glands were isolated and microscopically examined for the presence of sporozoites.

Results

In total, 33% (85/256) of mosquito pools tested positive for avian Plasmodium spp., harbouring at least eight distinct parasite lineages. Sporozoites of Plasmodium spp. were recorded in salivary glands of C. aurites supporting the PCR data that the parasites complete development in these mosquitoes. Results

SUBMITTER: Njabo KY 

PROVIDER: S-EPMC3152766 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature